Axxam’s Photoproteins : Photina® and i-Photina®
Axxam’s researchers developed two proprietary Ca2+ activated photoproteins optimized for the generation of precise Ca2+ mobilization assays for High-Throughput Screening (HTS) : Photina® and i-Photina®. The total light release of Axxam’s photoproteins is greater and the reaction kinetic is slower compared to other existing photoproteins, opening new opportunities for the application of flash luminescence assays in HTS.
Axxam scientists validated the expression and functionality of Photina® and i-Photina® in the cell lines most frequently used in HTS, such as CHO, HEK293 and Jurkat cells and using several flash luminescence plate readers such as - FLIPR3, FLIPRTETRA, CyBi® Lumax, Lumilux.
Photina® and i-Photina® are very well suited to measuring GPCR activation and are ideal for any other cellular targets capable of increasing the intracellular Ca2+ concentration such as Ca2+ permeable ion channels or Na+/Ca2+ exchangers.
In June 2010 Axxam and Lonza announced the signing of a license agreement enabling Lonza to include Axxam’s Photina®, i-Photina® and Clytin® Photoproteins as calcium biosensors in Lonza’s broad range of Clonetics® Primary Cells. (http://www.lonzabio.com/drug-discovery/clonetics-primary-sensors)
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contact us to receive further information on Axxam’s proprietary photoproteins.
chAMPion: Universal Reporter Cell Line
The chAMPion cellular reporter system is a novel combination of technology coupled with the sensitivity of using cyclic nucleotide gated channels for measurement of cAMP levels.
chAMPion technology is based on the co-expression of one of Axxam’s proprietary Ca2+ activated photoproteins and a cyclic nucleotide-gated (CNG) channel acting as a cAMP biosensor in a CHO-K1 cell line.
chAMPion technology represents a platform that permits interrogation of all the major classes of coupled G-protein coupled receptors, Gq, Gi and Gs, as the adenylate cylcase/cAMP modulated pathway is also linked to calcium flux via the CNG sensor, and the Gq/PLC pathway naturally induces changes in calcium levels, both of which can be monitored by activation of the calcium-sensitive photoprotein light production.
The chAMPion cell line displays many advantages:
- a real-time assay for sensitive detection of cGMP and cAMP
- suitable for HTS and uHTS
- requires short incubation times decreasing the false positives rate
- avoids any fluorescence interference from test compounds
- enables the characterization of GPCRs coupled to all the different Gα proteins
- assay platform for different multiple target classes, such as:
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contact us to receive further information on chAMPion Technology |
